BLASTP 2.2.23 [Feb-03-2010]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Reference for compositional score matrix adjustment:
Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala,
Aleksandr Morgulis, Alejandro A. Schäffer, and Yi-Kuo Yu (2005) "Protein database
searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109.

Query= Os02g0257300 Os02g0257300|Os02g0257300
         (478 letters)

Database: tair10 
           27,416 sequences; 11,106,569 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

AT4G01050.1  | chr4:455874-458175 FORWARD LENGTH=467              260   9e-70
AT3G25480.1  | chr3:9235591-9236598 REVERSE LENGTH=265            100   2e-21
AT5G23060.1  | chr5:7736760-7738412 REVERSE LENGTH=388             51   1e-06

>AT4G01050.1 | chr4:455874-458175 FORWARD LENGTH=467
          Length = 466

 Score =  260 bits (665), Expect = 9e-70,   Method: Compositional matrix adjust.
 Identities = 139/236 (58%), Positives = 168/236 (71%), Gaps = 10/236 (4%)

Query: 126 KPYGVVSAAAAYRALVEEPGAQLVDIRPPGDARQSGAPDLREAKKKAAAVPYDGEDKNGF 185
           K +GV SA  AY  L  +  AQL+DIR   D RQ G+P+++   KKA +  Y+GEDK GF
Sbjct: 128 KSWGVESAKNAYTKLGTDDNAQLLDIRATADFRQVGSPNIKGLGKKAVSTVYNGEDKPGF 187

Query: 186 LKKLSLRFKDPENTTLVILDKFDGNSELVAELVTANGYKAAFAVKDGAEGRRGWLSSSLP 245
           LKKLSL+FKDPENTTL ILDKFDGNSELVAELV  NG+K+A+A+KDGAEG RGWL+SSLP
Sbjct: 188 LKKLSLKFKDPENTTLYILDKFDGNSELVAELVALNGFKSAYAIKDGAEGPRGWLNSSLP 247

Query: 246 WTAPKKGFSL---------SDLIGDGTDGLPVXXXXXXXXXXXXXXXXEIETVLQFLGSA 296
           W  PKK  SL         S + G+ +DG+ V                EIET+LQ LGSA
Sbjct: 248 WIEPKKTLSLDLSSLTDSISGVFGESSDGVSVALGVAAAAGLSVFAFTEIETILQLLGSA 307

Query: 297 AIVQLVASKLIYAEDRKRTLKQIDDFFNKKVAPKELVDEIKEIGQALLP-STGTKS 351
           A+VQL   KL++AEDRK+TLKQ+D+F N KVAPKELVDE+KEIG+ALLP ST  K+
Sbjct: 308 ALVQLAGKKLLFAEDRKQTLKQVDEFLNTKVAPKELVDELKEIGKALLPQSTSNKA 363



>AT3G25480.1 | chr3:9235591-9236598 REVERSE LENGTH=265
          Length = 264

 Score =  100 bits (248), Expect = 2e-21,   Method: Compositional matrix adjust.
 Identities = 48/116 (41%), Positives = 69/116 (59%)

Query: 126 KPYGVVSAAAAYRALVEEPGAQLVDIRPPGDARQSGAPDLREAKKKAAAVPYDGEDKNGF 185
           + Y  +SA  A+R L  E  +QL+DIR         +P+L+   K +  VP+   D+ GF
Sbjct: 116 RKYKPISAMNAFRKLKNESDSQLLDIRDVKTLALLASPNLKFLGKSSVQVPFSENDEEGF 175

Query: 186 LKKLSLRFKDPENTTLVILDKFDGNSELVAELVTANGYKAAFAVKDGAEGRRGWLS 241
           L K+   F D ENT + +LD FDGNS  VAEL+  NG+K A+ ++ GA G+ GWL+
Sbjct: 176 LTKVKGSFSDAENTVVCVLDNFDGNSSKVAELLIKNGFKEAYYIRGGARGKNGWLA 231



>AT5G23060.1 | chr5:7736760-7738412 REVERSE LENGTH=388
          Length = 387

 Score = 51.2 bits (121), Expect = 1e-06,   Method: Compositional matrix adjust.
 Identities = 33/112 (29%), Positives = 55/112 (49%), Gaps = 15/112 (13%)

Query: 148 LVDIRPPGDARQSGAPDL-REAKKKAAAVPYDG--EDKNGFLK------------KLSLR 192
           +VDIR   D  ++G P L   AK +  ++P +       G ++            K+S  
Sbjct: 237 MVDIRSEKDKEKAGIPRLPSNAKNRVISIPLEELPNKVKGIVRNSKRVEAEIAALKISYL 296

Query: 193 FKDPENTTLVILDKFDGNSELVAELVTANGYKAAFAVKDGAEGRRGWLSSSL 244
            K  + + ++ILD +  ++++VA+ +   GYK  + V DG  G RGWL S L
Sbjct: 297 KKINKGSNIIILDSYTDSAKIVAKTLKVLGYKNCYIVTDGFSGGRGWLQSRL 348



  Database: tair10
    Posted date:  Dec 8, 2010 11:30 AM
  Number of letters in database: 11,106,569
  Number of sequences in database:  27,416
  
Lambda     K      H
   0.313    0.134    0.377 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 27416
Number of Hits to DB: 5,713,098
Number of extensions: 194732
Number of successful extensions: 340
Number of sequences better than 1.0e-05: 3
Number of HSP's gapped: 339
Number of HSP's successfully gapped: 3
Length of query: 478
Length of database: 11,106,569
Length adjustment: 102
Effective length of query: 376
Effective length of database: 8,310,137
Effective search space: 3124611512
Effective search space used: 3124611512
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.2 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 42 (21.9 bits)
S2: 114 (48.5 bits)