BLASTP 2.2.23 [Feb-03-2010]
Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Reference for compositional score matrix adjustment:
Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala,
Aleksandr Morgulis, Alejandro A. Schäffer, and Yi-Kuo Yu (2005) "Protein database
searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109.
Query= Os02g0169700 Os02g0169700|AK105565
(146 letters)
Database: tair10
27,416 sequences; 11,106,569 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
AT2G35120.1 | chr2:14805913-14807274 REVERSE LENGTH=157 170 2e-43
AT1G32470.1 | chr1:11739479-11740246 REVERSE LENGTH=167 149 5e-37
AT2G35370.1 | chr2:14891239-14892050 FORWARD LENGTH=166 145 8e-36
>AT2G35120.1 | chr2:14805913-14807274 REVERSE LENGTH=157
Length = 156
Score = 170 bits (431), Expect = 2e-43, Method: Compositional matrix adjust.
Identities = 84/141 (59%), Positives = 109/141 (77%), Gaps = 1/141 (0%)
Query: 6 INLFQGATNLNVLKDLKYSETHEWVSVEGDYATIGISDHAQDHLGYVASVELPEVGQSVS 65
I++ Q + VLKDLKY+++HEWV ++G+ AT GI+DHAQDHLG V VELP+VG SVS
Sbjct: 17 ISVAQRGFSSVVLKDLKYADSHEWVKIDGNKATFGITDHAQDHLGDVVYVELPDVGHSVS 76
Query: 66 QGNKFGVVVSVKGTTRGINSPVSGEVVEVNDQLSDLPGLVNARPYETGWIIVVKISDSGE 125
QG FG V SVK T+ INSPVSG+VVEVN++L++ PGLVN+ PYE GWII V++SD+GE
Sbjct: 77 QGKSFGAVESVKATS-DINSPVSGKVVEVNEELTESPGLVNSSPYEQGWIIKVELSDAGE 135
Query: 126 LNSLMNDEDYSKFCKGEKDKY 146
LM+ + YSKFC+ E K+
Sbjct: 136 AEKLMDSDKYSKFCEEEDAKH 156
>AT1G32470.1 | chr1:11739479-11740246 REVERSE LENGTH=167
Length = 166
Score = 149 bits (376), Expect = 5e-37, Method: Compositional matrix adjust.
Identities = 73/127 (57%), Positives = 93/127 (73%), Gaps = 1/127 (0%)
Query: 16 NVLKDLKYSETHEWVSVEGDYATIGISDHAQDHLGYVASVELPEVGQSVSQGNKFGVVVS 75
+VL+ LKY+ +HEWV EG ATIGI+DHAQDHLG V VELPE SVS+ FG V S
Sbjct: 37 SVLEGLKYANSHEWVKHEGSVATIGITDHAQDHLGEVVFVELPEANSSVSKEKSFGAVES 96
Query: 76 VKGTTRGINSPVSGEVVEVNDQLSDLPGLVNARPYETGWIIVVKISDSGELNSLMNDEDY 135
VK T+ I SP+SGEV+EVN +L++ PGL+N+ PYE GW+I VK S EL +LM ++Y
Sbjct: 97 VKATSE-ILSPISGEVIEVNTKLTESPGLINSSPYEDGWMIKVKPSSPAELEALMGPKEY 155
Query: 136 SKFCKGE 142
+KFC+ E
Sbjct: 156 TKFCEEE 162
>AT2G35370.1 | chr2:14891239-14892050 FORWARD LENGTH=166
Length = 165
Score = 145 bits (366), Expect = 8e-36, Method: Compositional matrix adjust.
Identities = 72/127 (56%), Positives = 91/127 (71%), Gaps = 1/127 (0%)
Query: 16 NVLKDLKYSETHEWVSVEGDYATIGISDHAQDHLGYVASVELPEVGQSVSQGNKFGVVVS 75
VL+ LKY+ +HEWV EG ATIGI+ HAQDHLG V VELPE SVS+ FG V S
Sbjct: 36 TVLEGLKYANSHEWVKHEGSVATIGITAHAQDHLGEVVFVELPEDNTSVSKEKSFGAVES 95
Query: 76 VKGTTRGINSPVSGEVVEVNDQLSDLPGLVNARPYETGWIIVVKISDSGELNSLMNDEDY 135
VK T+ I SP+SGE++EVN +L++ PGL+N+ PYE GW+I VK S EL SLM ++Y
Sbjct: 96 VKATSE-ILSPISGEIIEVNKKLTESPGLINSSPYEDGWMIKVKPSSPAELESLMGPKEY 154
Query: 136 SKFCKGE 142
+KFC+ E
Sbjct: 155 TKFCEEE 161
Database: tair10
Posted date: Dec 8, 2010 11:30 AM
Number of letters in database: 11,106,569
Number of sequences in database: 27,416
Lambda K H
0.311 0.132 0.379
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 27416
Number of Hits to DB: 3,275,327
Number of extensions: 127367
Number of successful extensions: 283
Number of sequences better than 1.0e-05: 3
Number of HSP's gapped: 280
Number of HSP's successfully gapped: 3
Length of query: 146
Length of database: 11,106,569
Length adjustment: 89
Effective length of query: 57
Effective length of database: 8,666,545
Effective search space: 493993065
Effective search space used: 493993065
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.2 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 42 (21.7 bits)
S2: 107 (45.8 bits)