BLASTP 2.2.23 [Feb-03-2010]
Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Reference for compositional score matrix adjustment:
Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala,
Aleksandr Morgulis, Alejandro A. Schäffer, and Yi-Kuo Yu (2005) "Protein database
searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109.
Query= Os02g0141500 Os02g0141500|AK099428
(228 letters)
Database: tair10
27,416 sequences; 11,106,569 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
AT5G35320.1 | chr5:13521893-13523144 FORWARD LENGTH=226 70 8e-13
>AT5G35320.1 | chr5:13521893-13523144 FORWARD LENGTH=226
Length = 225
Score = 70.5 bits (171), Expect = 8e-13, Method: Compositional matrix adjust.
Identities = 74/252 (29%), Positives = 110/252 (43%), Gaps = 67/252 (26%)
Query: 8 KRKELDQVVDGLSDFSLSGPAAKSRRLDIGLPPIMEEETPGPSMPFGHEMLGEEIHSVVS 67
KRK++D+V D SDFSLS PA K RRLD+ LPPIMEEE +
Sbjct: 10 KRKDIDRVNDDFSDFSLSSPARKIRRLDVDLPPIMEEEE-------------------ID 50
Query: 68 MPIIEDMLGGAMESHPSSEDKALVLYKSVDNTACIGPGTSNPSIIISPDLIRSLKNQAFM 127
+P ++D + +E P ++++A+VL+K + P + N + + LI KN+
Sbjct: 51 LP-MQDTVAEEIELEPVNDERAIVLFKPLHYHQ---PSSGN--VFVDRHLISGFKNRFLR 104
Query: 128 QRNF---HGLEDKSPGCSNFLALVPWTPPQIATVSNWSASDSEKREFFEVPMESDETEVD 184
+ + ED+ + A+V W P Q S +S S F+ P + TE+D
Sbjct: 105 DVSIADDNQYEDER--LNKCQAVVCWNPSQ----STYSQSIGT----FQQPRTLEITELD 154
Query: 185 -----------SMDVEESPEANVAAI------------DGESLHQWQ--QHCMTPSSLPN 219
S ++EE + + G LH WQ Q+CM P LP
Sbjct: 155 ETGEDVVMDDASNEIEEDTGSTSLSFPQQGQQQEQEHTYGFGLHPWQQAQNCMIP-QLPQ 213
Query: 220 PS---APVMWSR 228
S P+ W R
Sbjct: 214 VSTTPTPITWFR 225
Database: tair10
Posted date: Dec 8, 2010 11:30 AM
Number of letters in database: 11,106,569
Number of sequences in database: 27,416
Lambda K H
0.313 0.130 0.389
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 27416
Number of Hits to DB: 5,830,716
Number of extensions: 258675
Number of successful extensions: 514
Number of sequences better than 1.0e-05: 1
Number of HSP's gapped: 514
Number of HSP's successfully gapped: 1
Length of query: 228
Length of database: 11,106,569
Length adjustment: 95
Effective length of query: 133
Effective length of database: 8,502,049
Effective search space: 1130772517
Effective search space used: 1130772517
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.2 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 42 (21.9 bits)
S2: 110 (47.0 bits)