BLASTP 2.2.23 [Feb-03-2010]
Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Reference for compositional score matrix adjustment:
Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala,
Aleksandr Morgulis, Alejandro A. Schäffer, and Yi-Kuo Yu (2005) "Protein database
searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109.
Query= Os01g0917300 Os01g0917300|J065179H19
(125 letters)
Database: tair10
27,416 sequences; 11,106,569 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
AT1G65295.1 | chr1:24251567-24252054 FORWARD LENGTH=116 96 6e-21
AT5G01015.1 | chr5:5335-5769 REVERSE LENGTH=105 59 5e-10
>AT1G65295.1 | chr1:24251567-24252054 FORWARD LENGTH=116
Length = 115
Score = 95.5 bits (236), Expect = 6e-21, Method: Compositional matrix adjust.
Identities = 49/84 (58%), Positives = 60/84 (71%), Gaps = 11/84 (13%)
Query: 42 MVPVVEGGGGFSAMVLNETRRRLGSFQLCAPCTCCXXXXXXXXXXXXXCILSPCCYAINC 101
MVP+ G MV+NETRR+LGSFQ+C+ CTCC C+ SPCCYAINC
Sbjct: 43 MVPL----GEEKFMVMNETRRKLGSFQICSVCTCCGGAKGL-------CLPSPCCYAINC 91
Query: 102 NIPNRPFGFCSFTPKSCDCLGCNL 125
NIPNRPFG+CSFTPK+C+C GC++
Sbjct: 92 NIPNRPFGYCSFTPKTCNCFGCHI 115
>AT5G01015.1 | chr5:5335-5769 REVERSE LENGTH=105
Length = 104
Score = 59.3 bits (142), Expect = 5e-10, Method: Compositional matrix adjust.
Identities = 36/101 (35%), Positives = 47/101 (46%), Gaps = 16/101 (15%)
Query: 26 ATSQVGGAGEEVEVARMVPVVEGGGGFSAMVLNETRRRLGS-FQLCAPCTCCXXXXXXXX 84
T+ G G + M PV G M RR+L + +LC C CC
Sbjct: 19 VTTNGQGDGTGLNAEEMWPVEVG------MEYRVWRRKLMTPLELCLECKCCSSTT---- 68
Query: 85 XXXXXCILSPCCYAINCNIPNRPFGFCSFTPKSCDCLGCNL 125
C PCC+ INC +PN+PFG C+F PKSC C C++
Sbjct: 69 -----CATMPCCFGINCQLPNKPFGVCAFVPKSCHCNSCSI 104
Database: tair10
Posted date: Dec 8, 2010 11:30 AM
Number of letters in database: 11,106,569
Number of sequences in database: 27,416
Lambda K H
0.327 0.141 0.494
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 27416
Number of Hits to DB: 2,118,436
Number of extensions: 63987
Number of successful extensions: 138
Number of sequences better than 1.0e-05: 2
Number of HSP's gapped: 136
Number of HSP's successfully gapped: 2
Length of query: 125
Length of database: 11,106,569
Length adjustment: 86
Effective length of query: 39
Effective length of database: 8,748,793
Effective search space: 341202927
Effective search space used: 341202927
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 15 ( 7.1 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 40 (21.7 bits)
S2: 105 (45.1 bits)