BLASTP 2.2.23 [Feb-03-2010]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Reference for compositional score matrix adjustment:
Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala,
Aleksandr Morgulis, Alejandro A. Schäffer, and Yi-Kuo Yu (2005) "Protein database
searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109.

Query= Os01g0851400 Os01g0851400|J065070P10
         (336 letters)

Database: tair10 
           27,416 sequences; 11,106,569 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

AT3G54130.1  | chr3:20042895-20044429 REVERSE LENGTH=281          311   4e-85

>AT3G54130.1 | chr3:20042895-20044429 REVERSE LENGTH=281
          Length = 280

 Score =  311 bits (796), Expect = 4e-85,   Method: Compositional matrix adjust.
 Identities = 164/258 (63%), Positives = 189/258 (73%), Gaps = 22/258 (8%)

Query: 8   SNGGLLYHEVQEGKLCAVHCVNTTLQGPFFSEFDLSALAVDLDQRERQVMSEXXXXXXXX 67
           SNGG+LYHEVQE  LCAVHCVNT LQGPFFSEFDL+A+A DLD +ERQVM E        
Sbjct: 5   SNGGMLYHEVQESNLCAVHCVNTVLQGPFFSEFDLAAVAADLDGKERQVMLEGAAVGGFA 64

Query: 68  XXXDFLAEGEGSHNVSLGGDFSIQVLQKALEVWDLQVIPLDSPDVGSCLFDPELETAFIC 127
              DFLAE   SHNVSLGGDFSIQVLQKALEVWDLQVIPL+ PD      DPELE+AFIC
Sbjct: 65  PG-DFLAEE--SHNVSLGGDFSIQVLQKALEVWDLQVIPLNCPDAEPAQIDPELESAFIC 121

Query: 128 HLQDHWFCIRKVNGEWYNFNSLYPAPEHLSKFYLSAFIDTLKGSGWSIFAVRGNFPKECP 187
           HL DHWFCIRKVNGEWYNF+SL  AP+HLSKFYLSAF+D+LKG+GWSIF V+GNFP+ECP
Sbjct: 122 HLHDHWFCIRKVNGEWYNFDSLLAAPQHLSKFYLSAFLDSLKGAGWSIFIVKGNFPQECP 181

Query: 188 M--ATEGSNGFGQWLTPDDARRI---TSSCNQVQTPT-------QQAGVSLVADQSEEMS 235
           M  ++E SN FGQWL+P+DA RI   TSS +  +          Q+   +L  ++ +  S
Sbjct: 182 MSSSSEASNSFGQWLSPEDAERIRKNTSSGSSARNKRSNDNVNQQRRNQALSREEVQAFS 241

Query: 236 EMDMIAAQQEEADLNAAI 253
           EM       E+ DL AAI
Sbjct: 242 EM-------EDDDLKAAI 252



  Database: tair10
    Posted date:  Dec 8, 2010 11:30 AM
  Number of letters in database: 11,106,569
  Number of sequences in database:  27,416
  
Lambda     K      H
   0.312    0.128    0.375 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 27416
Number of Hits to DB: 7,229,864
Number of extensions: 296851
Number of successful extensions: 800
Number of sequences better than 1.0e-05: 1
Number of HSP's gapped: 797
Number of HSP's successfully gapped: 1
Length of query: 336
Length of database: 11,106,569
Length adjustment: 99
Effective length of query: 237
Effective length of database: 8,392,385
Effective search space: 1988995245
Effective search space used: 1988995245
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.2 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 42 (21.9 bits)
S2: 112 (47.8 bits)