BLASTP 2.2.23 [Feb-03-2010]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Reference for compositional score matrix adjustment:
Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala,
Aleksandr Morgulis, Alejandro A. Schäffer, and Yi-Kuo Yu (2005) "Protein database
searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109.

Query= Os01g0743400 Os01g0743400|AK059177
         (393 letters)

Database: tair10 
           27,416 sequences; 11,106,569 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

AT2G25840.2  | chr2:11021924-11025158 FORWARD LENGTH=413          511   e-145

>AT2G25840.2 | chr2:11021924-11025158 FORWARD LENGTH=413
          Length = 412

 Score =  511 bits (1317), Expect = e-145,   Method: Compositional matrix adjust.
 Identities = 252/369 (68%), Positives = 286/369 (77%), Gaps = 17/369 (4%)

Query: 35  LRLNCSVAEATASGDEAPAPPARKKRVVSGVQPTGLVHLGNYLGAIKNWVSLQDLYETLF 94
            R  CSVA    S       P+ KKRVVSGVQPTG VHLGNYLGAIKNWV+LQD YETLF
Sbjct: 48  FRCCCSVATDDTS-------PSVKKRVVSGVQPTGSVHLGNYLGAIKNWVALQDTYETLF 100

Query: 95  FIVDLHAITLPYEAPQLSKATRSTAAIYLACGIDSSK------SHVRAHVELMWLLSSST 148
            IVD HAITLPY+  QL KAT  TAA+YLACGID SK      SHV AHVELMWLL SST
Sbjct: 101 IIVDHHAITLPYDTRQLGKATTDTAALYLACGIDVSKASVFVQSHVPAHVELMWLLCSST 160

Query: 149 PIGWLNRMIQFKEKSRKAGDENVGVALLTYPVLMASDILLYQSDLVPVGEDQTQHLELTR 208
           PIGWL +MIQFKEKSRK G EN  V L TYP LM +DILLYQSD VPVGEDQ QH+EL R
Sbjct: 161 PIGWLQKMIQFKEKSRKEGVENASVGLFTYPDLMTADILLYQSDFVPVGEDQKQHIELAR 220

Query: 209 EIAERVNNLYXXXXXXXXXXXXX----SLFKVPEALIPPAGARVMSLTDGLSKMSKSAPS 264
           EIA+RVN+LY                 SLFK+PE LIP AGARVMSLTDGLSKMSKSAPS
Sbjct: 221 EIAQRVNHLYGGKKWKKLGGYEIFRGGSLFKIPEPLIPQAGARVMSLTDGLSKMSKSAPS 280

Query: 265 DQSRINLLDPKDVIMNKIKRCKTDSFPGLEFDNPERPECNNLLSIYQIITGKTKEEVIRE 324
           DQSRINLLD KD+I++KIKRCKTDSF GLEFDN ERPECNNLLSIYQI++GK KEEV+ E
Sbjct: 281 DQSRINLLDSKDLIVDKIKRCKTDSFAGLEFDNAERPECNNLLSIYQIVSGKKKEEVMEE 340

Query: 325 CENMNWGSFKTTLTDALIDHLQPIQVRYEEIMSDPGYLDGVLLEGSGKAAEIADITLNNV 384
           C++M+WG+FK  L DALI+HL PIQ RY+EI+++P YLD +L EG+ +A E+  +T+ N+
Sbjct: 341 CKDMSWGTFKPLLADALIEHLSPIQARYQEIIAEPEYLDKILSEGADRAEELGAVTMRNM 400

Query: 385 YQAMGFLRR 393
           YQAMG+ +R
Sbjct: 401 YQAMGYYQR 409



  Database: tair10
    Posted date:  Dec 8, 2010 11:30 AM
  Number of letters in database: 11,106,569
  Number of sequences in database:  27,416
  
Lambda     K      H
   0.318    0.134    0.388 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 27416
Number of Hits to DB: 7,509,193
Number of extensions: 292926
Number of successful extensions: 670
Number of sequences better than 1.0e-05: 1
Number of HSP's gapped: 668
Number of HSP's successfully gapped: 1
Length of query: 393
Length of database: 11,106,569
Length adjustment: 101
Effective length of query: 292
Effective length of database: 8,337,553
Effective search space: 2434565476
Effective search space used: 2434565476
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
S2: 113 (48.1 bits)