BLASTP 2.2.23 [Feb-03-2010]
Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Reference for compositional score matrix adjustment:
Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala,
Aleksandr Morgulis, Alejandro A. Schäffer, and Yi-Kuo Yu (2005) "Protein database
searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109.
Query= Os01g0734100 Os01g0734100|AK064618
(122 letters)
Database: tair10
27,416 sequences; 11,106,569 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
AT1G16740.1 | chr1:5727773-5728950 FORWARD LENGTH=127 209 2e-55
ATCG00660.1 | chrC:68512-68865 REVERSE LENGTH=118 61 1e-10
>AT1G16740.1 | chr1:5727773-5728950 FORWARD LENGTH=127
Length = 126
Score = 209 bits (533), Expect = 2e-55, Method: Compositional matrix adjust.
Identities = 100/114 (87%), Positives = 107/114 (93%)
Query: 1 MNKGKIFKLAKGFRGRAKNCIRIARERVEKALQYSYRDRHNKKRDMRSLWIERINAGTRL 60
MNK +IFKLAKGFRGRAKNCIRIARERVEKALQYSYRDR NKKR+MR LWIERINAG+R
Sbjct: 1 MNKKEIFKLAKGFRGRAKNCIRIARERVEKALQYSYRDRRNKKREMRGLWIERINAGSRQ 60
Query: 61 HGVNYGNFMHGLMKENIQLNRKVLSELSMHEPYSFKALVDVSRSAFPGNRPPVK 114
HGVNYGNF+HGLMKENIQLNRKVLSELSMHEPYSFKALVDVSR +FPGN+ V+
Sbjct: 61 HGVNYGNFIHGLMKENIQLNRKVLSELSMHEPYSFKALVDVSRKSFPGNKNVVQ 114
>ATCG00660.1 | chrC:68512-68865 REVERSE LENGTH=118
Length = 117
Score = 61.2 bits (147), Expect = 1e-10, Method: Compositional matrix adjust.
Identities = 34/89 (38%), Positives = 52/89 (58%), Gaps = 2/89 (2%)
Query: 3 KGKIFKLAKGFRGRAKNCIRIARERVEKALQYSYRDRHNKKRDMRSLWIERINAGTRLHG 62
+ K+ A FRG R ++ +AL ++RDR +KRD R LWI RINA G
Sbjct: 14 RTKLRLFASSFRGAHSRLTRTMTQQRIRALVSAHRDRGKRKRDFRRLWITRINAVIHEMG 73
Query: 63 V--NYGNFMHGLMKENIQLNRKVLSELSM 89
V +Y F+H L K+ + LNRK+L+++++
Sbjct: 74 VFYSYNEFIHNLYKKQLLLNRKILAQIAL 102
Database: tair10
Posted date: Dec 8, 2010 11:30 AM
Number of letters in database: 11,106,569
Number of sequences in database: 27,416
Lambda K H
0.322 0.137 0.402
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 27416
Number of Hits to DB: 2,536,450
Number of extensions: 88154
Number of successful extensions: 264
Number of sequences better than 1.0e-05: 2
Number of HSP's gapped: 263
Number of HSP's successfully gapped: 2
Length of query: 122
Length of database: 11,106,569
Length adjustment: 86
Effective length of query: 36
Effective length of database: 8,748,793
Effective search space: 314956548
Effective search space used: 314956548
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.9 bits)
S2: 105 (45.1 bits)