BLASTP 2.2.23 [Feb-03-2010]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Reference for compositional score matrix adjustment:
Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala,
Aleksandr Morgulis, Alejandro A. Schäffer, and Yi-Kuo Yu (2005) "Protein database
searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109.

Query= Os01g0732000 Os01g0732000|AK062498
         (70 letters)

Database: tair10 
           27,416 sequences; 11,106,569 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

AT5G41685.1  | chr5:16669419-16669646 REVERSE LENGTH=76            62   4e-11
AT1G64220.1  | chr1:23833088-23833321 REVERSE LENGTH=78            57   2e-09

>AT5G41685.1 | chr5:16669419-16669646 REVERSE LENGTH=76
          Length = 75

 Score = 62.4 bits (150), Expect = 4e-11,   Method: Compositional matrix adjust.
 Identities = 27/36 (75%), Positives = 31/36 (86%)

Query: 35 MKNAKVVAHYGFIPLVILIGMNSEPKPRLAQLLSPI 70
          +K AKVV HYGFIPLVI +GMNS+PKP L QLLSP+
Sbjct: 40 LKKAKVVTHYGFIPLVIFVGMNSDPKPHLFQLLSPV 75



>AT1G64220.1 | chr1:23833088-23833321 REVERSE LENGTH=78
          Length = 77

 Score = 57.0 bits (136), Expect = 2e-09,   Method: Compositional matrix adjust.
 Identities = 24/36 (66%), Positives = 30/36 (83%)

Query: 35 MKNAKVVAHYGFIPLVILIGMNSEPKPRLAQLLSPI 70
          ++ AKV  HYGFIPL+I+IGMNS+PKP L  LLSP+
Sbjct: 42 LQKAKVATHYGFIPLIIIIGMNSDPKPHLFHLLSPV 77



  Database: tair10
    Posted date:  Dec 8, 2010 11:30 AM
  Number of letters in database: 11,106,569
  Number of sequences in database:  27,416
  
Lambda     K      H
   0.325    0.141    0.409 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 27416
Number of Hits to DB: 735,974
Number of extensions: 13781
Number of successful extensions: 40
Number of sequences better than 1.0e-05: 2
Number of HSP's gapped: 40
Number of HSP's successfully gapped: 2
Length of query: 70
Length of database: 11,106,569
Length adjustment: 42
Effective length of query: 28
Effective length of database: 9,955,097
Effective search space: 278742716
Effective search space used: 278742716
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 15 ( 7.0 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 40 (21.6 bits)
S2: 104 (44.7 bits)