BLASTP 2.2.23 [Feb-03-2010]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Reference for compositional score matrix adjustment:
Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala,
Aleksandr Morgulis, Alejandro A. Schäffer, and Yi-Kuo Yu (2005) "Protein database
searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109.

Query= Os01g0654200 Os01g0654200|AK100750
         (331 letters)

Database: tair10 
           27,416 sequences; 11,106,569 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

AT4G20310.2  | chr4:10961639-10963690 FORWARD LENGTH=489          178   4e-45

>AT4G20310.2 | chr4:10961639-10963690 FORWARD LENGTH=489
          Length = 488

 Score =  178 bits (451), Expect = 4e-45,   Method: Compositional matrix adjust.
 Identities = 108/307 (35%), Positives = 160/307 (52%), Gaps = 45/307 (14%)

Query: 18  LMITGVPEASPLSGYLSAHNFILSVDGLNITRADEWMKM---LTQDNVVQISSRDLLEGS 74
           L +  VP  SPL GYLS  + I+S+DG+ + +  EW+++   L ++N  + S+  L  G 
Sbjct: 210 LTVVDVPSVSPLFGYLSPGDVIVSLDGIQVHKPSEWLELAAILDKEN-SKTSNGSLYLG- 267

Query: 75  EGYRATGSRKGYCVPNSWMDASKNLWQINDKLSCPDDLVAFQRMSEKGIGKKEVEDKYCL 134
            G R     KGYCVP S ++       + ++  CP DL AF+ M       +EV    CL
Sbjct: 268 -GSRRFHHGKGYCVPISLIEEGYKGKMVENQFVCPGDLTAFRTMPCSNAAIREV--SVCL 324

Query: 135 IAKDVVKLKKCGNGWRGAE--DGRSNFACLEDEYCLVPVLGPGISWIEISYARPYSLECL 192
            AKD+VKL+KCG+GW      D +S+  C + + CL  +  PG+ W EI+Y R  S +C 
Sbjct: 325 DAKDIVKLQKCGDGWVTTSDTDNQSDCVCPQGDLCLQAMQSPGVLWTEITYKRTSSQDC- 383

Query: 193 QKERNSSLLHDGNNNPGLGPCQGTFVYAGDLLSAAHSIKLSSYRPRWPLLLFIADVPRIL 252
                S L+H                             L++Y+PRW    F    P IL
Sbjct: 384 -----SRLVH-----------------------------LTAYQPRWLFNFFGKSFPNIL 409

Query: 253 QDGLSCLFRVSAALAVVNCLPVYFLDGEAILETMLSYFSWFTRRQQRNILKVCRFLWTIL 312
           +  L+C F VS AL ++N LPVY+LDGE+ILE+ L  F+W + R+++  L+VC    ++L
Sbjct: 410 ERSLTCTFHVSLALVLLNSLPVYYLDGESILESSLQSFTWLSPRKKKKALQVCLVGGSLL 469

Query: 313 SIVLFSR 319
           S + F R
Sbjct: 470 SFLAFFR 476



  Database: tair10
    Posted date:  Dec 8, 2010 11:30 AM
  Number of letters in database: 11,106,569
  Number of sequences in database:  27,416
  
Lambda     K      H
   0.323    0.139    0.437 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 27416
Number of Hits to DB: 7,442,308
Number of extensions: 324825
Number of successful extensions: 760
Number of sequences better than 1.0e-05: 1
Number of HSP's gapped: 757
Number of HSP's successfully gapped: 2
Length of query: 331
Length of database: 11,106,569
Length adjustment: 99
Effective length of query: 232
Effective length of database: 8,392,385
Effective search space: 1947033320
Effective search space used: 1947033320
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.9 bits)
S2: 112 (47.8 bits)