BLASTP 2.2.23 [Feb-03-2010]
Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Reference for compositional score matrix adjustment:
Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala,
Aleksandr Morgulis, Alejandro A. Schäffer, and Yi-Kuo Yu (2005) "Protein database
searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109.
Query= Os01g0640700 Os01g0640700|J075057J21
(87 letters)
Database: tair10
27,416 sequences; 11,106,569 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
AT2G36330.1 | chr2:15233620-15235298 FORWARD LENGTH=284 55 1e-08
AT5G62820.1 | chr5:25223828-25224898 REVERSE LENGTH=298 52 4e-08
>AT2G36330.1 | chr2:15233620-15235298 FORWARD LENGTH=284
Length = 283
Score = 54.7 bits (130), Expect = 1e-08, Method: Compositional matrix adjust.
Identities = 28/62 (45%), Positives = 40/62 (64%)
Query: 25 QVLAYLLMXXXXXXXXRNDLWMSSFGKDPFNKKINSAVWFSFIAFIGLATNSLISTANLF 84
QVLAYLLM R D W+S++GKD F + ++++ SF+AF+ A +SLIS NLF
Sbjct: 219 QVLAYLLMSASTAAVTRVDDWVSNWGKDEFTEMASASIAMSFLAFLAFAFSSLISGYNLF 278
Query: 85 SM 86
+
Sbjct: 279 NQ 280
>AT5G62820.1 | chr5:25223828-25224898 REVERSE LENGTH=298
Length = 297
Score = 52.4 bits (124), Expect = 4e-08, Method: Compositional matrix adjust.
Identities = 28/70 (40%), Positives = 44/70 (62%), Gaps = 1/70 (1%)
Query: 17 HLIFLVSL-QVLAYLLMXXXXXXXXRNDLWMSSFGKDPFNKKINSAVWFSFIAFIGLATN 75
H +F+ S+ Q+LAYLLM R D W+S++GKD F + +++ SF+AF A +
Sbjct: 224 HDLFVFSMDQLLAYLLMSASSCAATRVDDWVSNWGKDEFTQMATASIAVSFLAFGAFAVS 283
Query: 76 SLISTANLFS 85
+LIS+ LF+
Sbjct: 284 ALISSYRLFT 293
Database: tair10
Posted date: Dec 8, 2010 11:30 AM
Number of letters in database: 11,106,569
Number of sequences in database: 27,416
Lambda K H
0.328 0.137 0.409
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 27416
Number of Hits to DB: 1,510,362
Number of extensions: 41039
Number of successful extensions: 100
Number of sequences better than 1.0e-05: 2
Number of HSP's gapped: 100
Number of HSP's successfully gapped: 2
Length of query: 87
Length of database: 11,106,569
Length adjustment: 58
Effective length of query: 29
Effective length of database: 9,516,441
Effective search space: 275976789
Effective search space used: 275976789
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 15 ( 7.1 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 40 (21.8 bits)
S2: 104 (44.7 bits)