BLASTP 2.2.23 [Feb-03-2010]
Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Reference for compositional score matrix adjustment:
Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala,
Aleksandr Morgulis, Alejandro A. Schäffer, and Yi-Kuo Yu (2005) "Protein database
searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109.
Query= Os01g0621300 Os01g0621300|J075014C24
(78 letters)
Database: tair10
27,416 sequences; 11,106,569 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
AT4G01090.1 | chr4:470834-473248 REVERSE LENGTH=715 110 2e-25
AT2G46380.1 | chr2:19039377-19042171 FORWARD LENGTH=769 107 9e-25
AT3G61670.1 | chr3:22819052-22821870 FORWARD LENGTH=791 107 2e-24
AT1G01440.1 | chr1:159935-162219 REVERSE LENGTH=665 99 4e-22
>AT4G01090.1 | chr4:470834-473248 REVERSE LENGTH=715
Length = 714
Score = 110 bits (274), Expect = 2e-25, Method: Compositional matrix adjust.
Identities = 49/75 (65%), Positives = 63/75 (84%)
Query: 4 PFIREFNYPMASNCASGDSGVFVNGRELHQRDLDLLVGRGLPRISGKSYSVEISGNITDE 63
PFI EF++PM NCA+G++ VFVNGRELH+RD +LLVGRGLPR +SY V+ISG I D+
Sbjct: 622 PFIEEFSHPMLDNCAAGNTDVFVNGRELHKRDFELLVGRGLPRDKNRSYIVDISGRILDQ 681
Query: 64 ETGKKLRSLGKLAPT 78
++G++L SLGKLAPT
Sbjct: 682 DSGEELHSLGKLAPT 696
>AT2G46380.1 | chr2:19039377-19042171 FORWARD LENGTH=769
Length = 768
Score = 107 bits (268), Expect = 9e-25, Method: Compositional matrix adjust.
Identities = 50/75 (66%), Positives = 58/75 (77%)
Query: 4 PFIREFNYPMASNCASGDSGVFVNGRELHQRDLDLLVGRGLPRISGKSYSVEISGNITDE 63
PFI E NYPM NCA G + VFVNGRELHQ+DL LL RGLPR +SY+V ISG + DE
Sbjct: 677 PFIEELNYPMPENCAGGTTRVFVNGRELHQKDLRLLTARGLPRDRDRSYTVYISGRVIDE 736
Query: 64 ETGKKLRSLGKLAPT 78
+TG++L SLGKLAPT
Sbjct: 737 DTGEELDSLGKLAPT 751
>AT3G61670.1 | chr3:22819052-22821870 FORWARD LENGTH=791
Length = 790
Score = 107 bits (266), Expect = 2e-24, Method: Compositional matrix adjust.
Identities = 48/75 (64%), Positives = 59/75 (78%)
Query: 4 PFIREFNYPMASNCASGDSGVFVNGRELHQRDLDLLVGRGLPRISGKSYSVEISGNITDE 63
PFI E NYPM NC+ G +GVFVNGRELH++DLDLL GRGLP +SY V+I+G + DE
Sbjct: 699 PFIEELNYPMPENCSGGTTGVFVNGRELHRKDLDLLAGRGLPPDRDRSYIVDITGRVIDE 758
Query: 64 ETGKKLRSLGKLAPT 78
+TG++L LGKLAPT
Sbjct: 759 DTGEELDCLGKLAPT 773
>AT1G01440.1 | chr1:159935-162219 REVERSE LENGTH=665
Length = 664
Score = 99.4 bits (246), Expect = 4e-22, Method: Compositional matrix adjust.
Identities = 43/75 (57%), Positives = 60/75 (80%)
Query: 4 PFIREFNYPMASNCASGDSGVFVNGRELHQRDLDLLVGRGLPRISGKSYSVEISGNITDE 63
PFI EF+ PM NC +G++ VFVNGRELH+RDL+LL RGLPR +SY ++I+G + D
Sbjct: 572 PFIEEFSRPMPDNCGAGNTSVFVNGRELHERDLELLSSRGLPRGKNRSYIIDIAGRVLDG 631
Query: 64 ETGKKLRSLGKLAPT 78
++G++L+SLG+LAPT
Sbjct: 632 DSGEELKSLGRLAPT 646
Database: tair10
Posted date: Dec 8, 2010 11:30 AM
Number of letters in database: 11,106,569
Number of sequences in database: 27,416
Lambda K H
0.315 0.136 0.389
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 27416
Number of Hits to DB: 1,763,246
Number of extensions: 65813
Number of successful extensions: 122
Number of sequences better than 1.0e-05: 4
Number of HSP's gapped: 122
Number of HSP's successfully gapped: 4
Length of query: 78
Length of database: 11,106,569
Length adjustment: 50
Effective length of query: 28
Effective length of database: 9,735,769
Effective search space: 272601532
Effective search space used: 272601532
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 42 (21.9 bits)
S2: 104 (44.7 bits)