BLASTP 2.2.23 [Feb-03-2010]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Reference for compositional score matrix adjustment:
Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala,
Aleksandr Morgulis, Alejandro A. Schäffer, and Yi-Kuo Yu (2005) "Protein database
searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109.

Query= Os01g0588000 Os01g0588000|AK109917
         (269 letters)

Database: tair10 
           27,416 sequences; 11,106,569 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

AT5G04890.1  | chr5:1427217-1428390 FORWARD LENGTH=367             50   2e-06
AT2G27140.1  | chr2:11598496-11599264 REVERSE LENGTH=225           49   2e-06

>AT5G04890.1 | chr5:1427217-1428390 FORWARD LENGTH=367
          Length = 366

 Score = 49.7 bits (117), Expect = 2e-06,   Method: Compositional matrix adjust.
 Identities = 32/101 (31%), Positives = 52/101 (51%), Gaps = 12/101 (11%)

Query: 1   MAEQQQQQRDG------DFEPVYEWLDAGAHYLLRVNVPEFKKEELQV-HVDPAGRLTVR 53
           MA +QQQ+  G      DF P  EW D     +L +++  F KE+++V +V  +  + V 
Sbjct: 1   MAARQQQKGTGFGVQYEDFVPKSEWKDQPEATILNIDLTGFAKEQMKVTYVHSSKMIRVT 60

Query: 54  GQHGGL-----RLNKVFQLPPTCNLDAITGRLEASVLVLTV 89
           G+         R N+VF +P  C +D I G  + +VL +T+
Sbjct: 61  GERPLANRKWSRFNEVFTVPQNCLVDKIHGSFKNNVLTITM 101



>AT2G27140.1 | chr2:11598496-11599264 REVERSE LENGTH=225
          Length = 224

 Score = 49.3 bits (116), Expect = 2e-06,   Method: Compositional matrix adjust.
 Identities = 24/81 (29%), Positives = 42/81 (51%), Gaps = 5/81 (6%)

Query: 12 DFEPVYEWLDAGAHYLLRVNVPEFKKEELQVHVDPAGRLTVRGQHGG-----LRLNKVFQ 66
          +FEP+  W        L + +P F+KE+L+V V    +L V G         +R  K F 
Sbjct: 16 EFEPLSNWKTEQGFEALTIYLPGFRKEQLKVQVTTTRKLRVMGDRPAGANKWIRFRKEFP 75

Query: 67 LPPTCNLDAITGRLEASVLVL 87
          +PP  ++D+++ + E + LV+
Sbjct: 76 IPPNIDVDSVSAKFEGANLVV 96



  Database: tair10
    Posted date:  Dec 8, 2010 11:30 AM
  Number of letters in database: 11,106,569
  Number of sequences in database:  27,416
  
Lambda     K      H
   0.313    0.129    0.368 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 27416
Number of Hits to DB: 3,325,001
Number of extensions: 101324
Number of successful extensions: 188
Number of sequences better than 1.0e-05: 2
Number of HSP's gapped: 193
Number of HSP's successfully gapped: 2
Length of query: 269
Length of database: 11,106,569
Length adjustment: 97
Effective length of query: 172
Effective length of database: 8,447,217
Effective search space: 1452921324
Effective search space used: 1452921324
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.2 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 42 (21.9 bits)
S2: 111 (47.4 bits)