BLASTP 2.2.23 [Feb-03-2010]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Reference for compositional score matrix adjustment:
Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala,
Aleksandr Morgulis, Alejandro A. Schäffer, and Yi-Kuo Yu (2005) "Protein database
searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109.

Query= Os01g0581300 Os01g0581300|AK066182
         (540 letters)

Database: tair10 
           27,416 sequences; 11,106,569 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

AT5G57030.1  | chr5:23077398-23079818 FORWARD LENGTH=525          657   0.0  
AT3G10230.1  | chr3:3164340-3165845 REVERSE LENGTH=502            270   1e-72

>AT5G57030.1 | chr5:23077398-23079818 FORWARD LENGTH=525
          Length = 524

 Score =  657 bits (1694), Expect = 0.0,   Method: Compositional matrix adjust.
 Identities = 319/474 (67%), Positives = 369/474 (77%), Gaps = 9/474 (1%)

Query: 68  GVEVEFADEEDYVKGGGGELLYVQMQAXXXXXXXXXXXXXLLPIPDENSVLDLVIIGCGP 127
            V  +FADEED+VK GG E+L+VQMQ              L PI   +  LDLV+IGCGP
Sbjct: 59  AVREDFADEEDFVKAGGSEILFVQMQQNKDMDEQSKLVDKLPPISIGDGALDLVVIGCGP 118

Query: 128 AGLSLAAESAKKGLNVGLIGPDLPFTNNYGVWEDEFKDLGLESCIEHVWKDTIVYLDGNK 187
           AGL+LAAESAK GL VGLIGPDLPFTNNYGVWEDEF DLGL+ CIEHVW++TIVYLD +K
Sbjct: 119 AGLALAAESAKLGLKVGLIGPDLPFTNNYGVWEDEFNDLGLQKCIEHVWRETIVYLDDDK 178

Query: 188 PIMIGRAYGXXXXXXXXXXXXXXCYDAGVTYLSSKVDKIMESPDGHRVVCCEGDREVLCR 247
           PI IGRAYG              C ++GV+YLSSKVD I E+ DG R+V C+ +  + CR
Sbjct: 179 PITIGRAYGRVSRRLLHEELLRRCVESGVSYLSSKVDSITEASDGLRLVACDDNNVIPCR 238

Query: 248 LAIVASGAASGRLLEYEVGGPRVCVQTAYGVEVEVENNPYDPSLMVFMDYRDCFKDKFSH 307
           LA VASGAASG+LL+YEVGGPRVCVQTAYGVEVEVEN+PYDP  MVFMDYRD   +K   
Sbjct: 239 LATVASGAASGKLLQYEVGGPRVCVQTAYGVEVEVENSPYDPDQMVFMDYRDYTNEKVRS 298

Query: 308 PEQGNPTFLYAMPMSSTRIFFEETCLASKEAMPFDLLKKRLMSRLDAMGVHIRKVYEEEW 367
            E   PTFLYAMPM+ +R+FFEETCLASK+ MPFDLLK +LM RLD +G+ I K YEEEW
Sbjct: 299 LEAEYPTFLYAMPMTKSRLFFEETCLASKDVMPFDLLKTKLMLRLDTLGIRILKTYEEEW 358

Query: 368 SYIPVGGSLPNTDQKNLAFGAAASMVHPATGYSVVRSLSEAPRYASVISDILRNRVYPGE 427
           SYIPVGGSLPNT+QKNLAFGAAASMVHPATGYSVVRSLSEAP+YASVI++ILR       
Sbjct: 359 SYIPVGGSLPNTEQKNLAFGAAASMVHPATGYSVVRSLSEAPKYASVIAEILREET---- 414

Query: 428 YLPGTSQ-SSSPSMLAWRTLWPQERKRQRSFFLFGLALIIQLNNEGIQTFFETFFRLPKW 486
               T Q +S+ S  AW TLWP ERKRQR+FFLFGLALI+Q + EGI++FF TFFRLPKW
Sbjct: 415 ----TKQINSNISRQAWDTLWPPERKRQRAFFLFGLALIVQFDTEGIRSFFRTFFRLPKW 470

Query: 487 MWRGFLGSTLSSVDLILFAFYMFTIAPNQMRMNLVRHLLSDPTGSTMIKTYLTL 540
           MW+GFLGSTL+S DL+LFA YMF I+PN +R  L+ HL+SDPTG+TMIKTYL +
Sbjct: 471 MWQGFLGSTLTSGDLVLFALYMFVISPNNLRKGLINHLISDPTGATMIKTYLKV 524



>AT3G10230.1 | chr3:3164340-3165845 REVERSE LENGTH=502
          Length = 501

 Score =  270 bits (690), Expect = 1e-72,   Method: Compositional matrix adjust.
 Identities = 150/420 (35%), Positives = 228/420 (54%), Gaps = 16/420 (3%)

Query: 109 LPIPD--ENSVLDLVIIGCGPAGLSLAAESAKKGLNVGLI--GPDLPFTNNYGVWEDEFK 164
           LP+ D  ++ V+DL I+G GPAGL++A + ++ GL+V  I   P L + NNYGVW DEF+
Sbjct: 72  LPLYDTSKSQVVDLAIVGGGPAGLAVAQQVSEAGLSVCSIDPSPKLIWPNNYGVWVDEFE 131

Query: 165 DLGLESCIEHVWKDTIVYLDGNKPIMIGRAYGXXXXXXXXXXXXXXCYDAGVTYLSSKVD 224
            + L  C++  W   +VY+D      + R YG              C   GV +  SKV 
Sbjct: 132 AMDLLDCLDTTWSGAVVYVDEGVKKDLSRPYGRVNRKQLKSKMLQKCITNGVKFHQSKVT 191

Query: 225 KIMESPDGHRVVCCEGDREVLCRLAIVASGAASGRLLEYEVGGPRVCVQTAYGVEVEVEN 284
            ++       VVC +G   V  + ++V       R L           Q AYG+  EV+ 
Sbjct: 192 NVVHEEANSTVVCSDG---VKIQASVVLDATGFSRCLVQYDKPYNPGYQVAYGIVAEVDG 248

Query: 285 NPYDPSLMVFMDYRDCFKDKFSHPEQGN---PTFLYAMPMSSTRIFFEETCLASKEAMPF 341
           +P+D   MVFMD+RD   D +   ++ N   PTFLYAMP SS RIF EET L ++  +  
Sbjct: 249 HPFDVDKMVFMDWRDKHLDSYPELKERNSKIPTFLYAMPFSSNRIFLEETSLVARPGLRM 308

Query: 342 DLLKKRLMSRLDAMGVHIRKVYEEEWSYIPVGGSLPNTDQKNLAFGAAASMVHPATGYSV 401
           + +++R+ +RL  +G++++++ E+E   IP+GG LP   Q+ +  G  A MVHP+TGY V
Sbjct: 309 EDIQERMAARLKHLGINVKRIEEDERCVIPMGGPLPVLPQRVVGIGGTAGMVHPSTGYMV 368

Query: 402 VRSLSEAPRYASVISDILRNRVYPGEYLPGTSQSSSPSMLAWRTLWPQERKRQRSFFLFG 461
            R+L+ AP  A+ I        Y G     + +    S   WR LWP ER+RQR FF FG
Sbjct: 369 ARTLAAAPIVANAIVR------YLGSPSSNSLRGDQLSAEVWRDLWPIERRRQREFFCFG 422

Query: 462 LALIIQLNNEGIQTFFETFFRLPKWMWRGFLGSTLSSVDLILFAFYMFTIAPNQMRMNLV 521
           + ++++L+ +  + FF+ FF L    W GFL S L   +L++F   +F+ A N  R+ ++
Sbjct: 423 MDILLKLDLDATRRFFDAFFDLQPHYWHGFLSSRLFLPELLVFGLSLFSHASNTSRLEIM 482



  Database: tair10
    Posted date:  Dec 8, 2010 11:30 AM
  Number of letters in database: 11,106,569
  Number of sequences in database:  27,416
  
Lambda     K      H
   0.321    0.138    0.426 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 27416
Number of Hits to DB: 11,077,151
Number of extensions: 449308
Number of successful extensions: 999
Number of sequences better than 1.0e-05: 2
Number of HSP's gapped: 994
Number of HSP's successfully gapped: 2
Length of query: 540
Length of database: 11,106,569
Length adjustment: 103
Effective length of query: 437
Effective length of database: 8,282,721
Effective search space: 3619549077
Effective search space used: 3619549077
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.9 bits)
S2: 114 (48.5 bits)