BLASTP 2.2.23 [Feb-03-2010]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Reference for compositional score matrix adjustment:
Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala,
Aleksandr Morgulis, Alejandro A. Schäffer, and Yi-Kuo Yu (2005) "Protein database
searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109.

Query= Os01g0259900 Os01g0259900|Os01g0259900
         (252 letters)

Database: tair10 
           27,416 sequences; 11,106,569 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

AT5G64680.3  | chr5:25854433-25855331 FORWARD LENGTH=212           64   1e-10

>AT5G64680.3 | chr5:25854433-25855331 FORWARD LENGTH=212
          Length = 211

 Score = 63.5 bits (153), Expect = 1e-10,   Method: Compositional matrix adjust.
 Identities = 42/108 (38%), Positives = 52/108 (48%), Gaps = 15/108 (13%)

Query: 22  EAMLDISQTESTELWLIQWPLNQLDASDFHGQELTLKLH----------RDXXXXXXXXX 71
           E + D   T S E WLIQ PL Q        +E TLK+           +D         
Sbjct: 17  EQLDDTDLTRSDEFWLIQAPLGQFP----EIEENTLKIEPDKDGLFGEFKDSNAGSLHLC 72

Query: 72  XXXXYDLVSFAAQQPDATVFLPSGPEAKAVGKIARRVSLVRYPDPEEL 119
               YDL SF +Q   A + +PS  E+  VGKI RRV+LVRYP+P EL
Sbjct: 73  LGAKYDLASFHSQDAGAELIIPS-EESMIVGKITRRVALVRYPEPNEL 119



  Database: tair10
    Posted date:  Dec 8, 2010 11:30 AM
  Number of letters in database: 11,106,569
  Number of sequences in database:  27,416
  
Lambda     K      H
   0.312    0.131    0.381 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 27416
Number of Hits to DB: 4,553,431
Number of extensions: 145071
Number of successful extensions: 256
Number of sequences better than 1.0e-05: 1
Number of HSP's gapped: 254
Number of HSP's successfully gapped: 1
Length of query: 252
Length of database: 11,106,569
Length adjustment: 96
Effective length of query: 156
Effective length of database: 8,474,633
Effective search space: 1322042748
Effective search space used: 1322042748
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.2 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 42 (21.8 bits)
S2: 110 (47.0 bits)