BLASTP 2.2.23 [Feb-03-2010]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Reference for compositional score matrix adjustment:
Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala,
Aleksandr Morgulis, Alejandro A. Schäffer, and Yi-Kuo Yu (2005) "Protein database
searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109.

Query= Os01g0259600 Os01g0259600|AK066193
         (369 letters)

Database: tair10 
           27,416 sequences; 11,106,569 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

AT5G03430.1  | chr5:849237-852867 REVERSE LENGTH=498              474   e-134

>AT5G03430.1 | chr5:849237-852867 REVERSE LENGTH=498
          Length = 497

 Score =  474 bits (1219), Expect = e-134,   Method: Compositional matrix adjust.
 Identities = 226/351 (64%), Positives = 276/351 (78%), Gaps = 5/351 (1%)

Query: 11  VGQEQFSPSSPGWPPFMRVNPILDWSYRDVWSFLLTCKVKYCSLYDQGYTSIGSIYDTVP 70
           VGQEQFSPSSPGWPPFMRVNPILDWSYRDVW+FLLTCKVKYCSLYDQGYTSIGSI+DTVP
Sbjct: 142 VGQEQFSPSSPGWPPFMRVNPILDWSYRDVWAFLLTCKVKYCSLYDQGYTSIGSIHDTVP 201

Query: 71  NALLC--DSTTGKSFRPAYMLSDGRLERAGRTKKNISSVSSNGTNSTEVEQTISRSASII 128
           N+LL   D+++ + F+PAY+LSDGRLERAGR KK  S      T S + E  +   AS+I
Sbjct: 202 NSLLSVNDTSSKEKFKPAYLLSDGRLERAGRVKKIASLKKDVDTESQKHEVLL---ASVI 258

Query: 129 VVGDEILFGTVEDKLGAGLCKKLHAIGWRVSHVAVVSNEIDSVAEEVERCKSTDDMVFLV 188
            VGDEIL GTVED+LG  LCKKL ++GW V    V+ N+IDSV+EEV+R +ST DMVF+ 
Sbjct: 259 AVGDEILSGTVEDQLGLSLCKKLTSVGWSVQQTTVLRNDIDSVSEEVDRQRSTSDMVFIY 318

Query: 189 GGLGPLHSDISLAGVAKAFGVRLAPDEEFEEYLSQLIGDNYTGDRNEMALLPEGITELLH 248
           GG+GPLHSD++LAGVAKAFGVRLAPDEEFEEYL  LI D  TGDRNEMA LPEGITELLH
Sbjct: 319 GGVGPLHSDVTLAGVAKAFGVRLAPDEEFEEYLRHLISDQCTGDRNEMAQLPEGITELLH 378

Query: 249 HKMLPLPLIKCKNVVILAATNVDELETEWGCLLDTQESGLVMAKSFVSKHLCTSLLDVKI 308
           H+ L +PLIKC+NV++LAATN +ELE EW CL +  + G      + S+ L TSL DV++
Sbjct: 379 HEKLSVPLIKCRNVIVLAATNTEELEKEWECLTELTKLGGGSLIEYSSRRLMTSLTDVEV 438

Query: 309 APVVAKLCIDFSDVYIGCYRISRSGPLVVSFIGKDNQRVEAAAEKLTNSFE 359
           A  ++KL ++F D+Y+GCYR SR GP+++   GKDN R+++AA+ L   F+
Sbjct: 439 AEPLSKLGLEFPDIYLGCYRKSRQGPIIICLTGKDNARMDSAAQALRKKFK 489



  Database: tair10
    Posted date:  Dec 8, 2010 11:30 AM
  Number of letters in database: 11,106,569
  Number of sequences in database:  27,416
  
Lambda     K      H
   0.319    0.135    0.402 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 27416
Number of Hits to DB: 8,210,627
Number of extensions: 349351
Number of successful extensions: 830
Number of sequences better than 1.0e-05: 1
Number of HSP's gapped: 828
Number of HSP's successfully gapped: 1
Length of query: 369
Length of database: 11,106,569
Length adjustment: 100
Effective length of query: 269
Effective length of database: 8,364,969
Effective search space: 2250176661
Effective search space used: 2250176661
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
S2: 112 (47.8 bits)