BLASTP 2.2.23 [Feb-03-2010]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Reference for compositional score matrix adjustment:
Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala,
Aleksandr Morgulis, Alejandro A. Schäffer, and Yi-Kuo Yu (2005) "Protein database
searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109.

Query= Os01g0253400 Os01g0253400|AK108904
         (297 letters)

Database: tair10 
           27,416 sequences; 11,106,569 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

AT5G49320.1  | chr5:19995083-19996214 REVERSE LENGTH=258          121   4e-28

>AT5G49320.1 | chr5:19995083-19996214 REVERSE LENGTH=258
          Length = 257

 Score =  121 bits (303), Expect = 4e-28,   Method: Compositional matrix adjust.
 Identities = 71/182 (39%), Positives = 102/182 (56%), Gaps = 24/182 (13%)

Query: 43  GIVGFILCLAAEGSRSEASHYLMTVXXXXXXXXXXXXQVDVCFYNSSGRAPLAFAIGAFL 102
           G+  F+LCL AE +RS+A+    T                 C YN SG+ PL     AF+
Sbjct: 39  GLSCFVLCLYAEATRSQATWGSKT-----------------CVYNGSGKTPLLCGAIAFV 81

Query: 103 MLAVAMFAEHAYMLLAVAAPDSSAAGLAVAEGHP-RVPSDPARLTWQTCCLFFVTWICFG 161
            LAVAM   H Y+L+AV    +++  L + E  P  VP+   RLT+Q    F  TW+CFG
Sbjct: 82  GLAVAMVGFHMYLLIAV----TTSPSLVLVEWDPDSVPAK--RLTFQAAFFFVSTWVCFG 135

Query: 162 LAEVMLMVGIAVESGHVSDWRKPRAVCHRVRPGVFAAAGILGLITXXXXXXXXXTALQTN 221
           + EV+L+V ++VESGH+ +W KP+  C  +R G+F+AAG+  LIT         TALQ +
Sbjct: 136 VGEVLLLVALSVESGHLKNWSKPKPSCLVIRQGLFSAAGVFSLITVFLATGLYLTALQAH 195

Query: 222 KL 223
           ++
Sbjct: 196 RI 197



  Database: tair10
    Posted date:  Dec 8, 2010 11:30 AM
  Number of letters in database: 11,106,569
  Number of sequences in database:  27,416
  
Lambda     K      H
   0.327    0.135    0.427 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 27416
Number of Hits to DB: 4,043,343
Number of extensions: 113445
Number of successful extensions: 240
Number of sequences better than 1.0e-05: 1
Number of HSP's gapped: 241
Number of HSP's successfully gapped: 1
Length of query: 297
Length of database: 11,106,569
Length adjustment: 98
Effective length of query: 199
Effective length of database: 8,419,801
Effective search space: 1675540399
Effective search space used: 1675540399
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 15 ( 7.1 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 40 (21.7 bits)
S2: 111 (47.4 bits)