BLASTP 2.2.23 [Feb-03-2010]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Reference for compositional score matrix adjustment:
Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala,
Aleksandr Morgulis, Alejandro A. Schäffer, and Yi-Kuo Yu (2005) "Protein database
searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109.

Query= Os01g0234700 Os01g0234700|AK108936
         (259 letters)

Database: tair10 
           27,416 sequences; 11,106,569 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

AT4G23930.1  | chr4:12436131-12436694 REVERSE LENGTH=188          108   3e-24
AT1G64450.1  | chr1:23941758-23942786 REVERSE LENGTH=343           81   4e-16

>AT4G23930.1 | chr4:12436131-12436694 REVERSE LENGTH=188
          Length = 187

 Score =  108 bits (270), Expect = 3e-24,   Method: Compositional matrix adjust.
 Identities = 56/163 (34%), Positives = 86/163 (52%), Gaps = 9/163 (5%)

Query: 97  RPRPPDIAVAAVRLPAFASGPNGTVAFTFEQTAAVRNPNRAPLAHFDSSLRVAYAGGELG 156
           RPR P+I+V +V++P+F S  N +V+FTF Q +AVRNPNRA  +H+++ +++ Y G  +G
Sbjct: 34  RPRDPEISVTSVKVPSF-SVANSSVSFTFSQFSAVRNPNRAAFSHYNNVIQLFYYGNRIG 92

Query: 157 SVYIPAGLIDGGRTKDMXXXXXXXXXXXXXXXXLPXXXXXXXXXXXXXXXXXXXXXXVME 216
             ++PAG I+ GRTK M                 P                       +E
Sbjct: 93  YTFVPAGEIESGRTKRM--------LATFSVQSFPLAAASSSQISAAQFQNSDRSGSTVE 144

Query: 217 VDSLLVVKGRVTVLRVLTHHVEAAKVCRVGVSPVDGKVLGFRC 259
           ++S L + GRV VL + TH + A   CR+ +S  DG ++  RC
Sbjct: 145 IESKLEMAGRVRVLGLFTHRIAAKCNCRIAISSSDGSIVAVRC 187



>AT1G64450.1 | chr1:23941758-23942786 REVERSE LENGTH=343
          Length = 342

 Score = 81.3 bits (199), Expect = 4e-16,   Method: Compositional matrix adjust.
 Identities = 39/77 (50%), Positives = 56/77 (72%), Gaps = 1/77 (1%)

Query: 97  RPRPPDIAVAAVRLPAFASGPNGTVAFTFEQTAAVRNPNRAPLAHFDSSLRVAYAGGELG 156
           +P+ P I+V AV+LP+FA   N T  F+F Q  AVRNPNRA  +H+DSS+++ Y+G ++G
Sbjct: 42  KPKDPKISVNAVQLPSFAVS-NNTANFSFSQYVAVRNPNRAVFSHYDSSIQLLYSGNQVG 100

Query: 157 SVYIPAGLIDGGRTKDM 173
            ++IPAG ID GR + M
Sbjct: 101 FMFIPAGKIDSGRIQYM 117




 Score = 56.2 bits (134), Expect = 2e-08,   Method: Compositional matrix adjust.
 Identities = 26/46 (56%), Positives = 30/46 (65%)

Query: 214 VMEVDSLLVVKGRVTVLRVLTHHVEAAKVCRVGVSPVDGKVLGFRC 259
            ME++S + + GRV VL V THHV A   CRV VS  DG VLGF C
Sbjct: 297 TMEIESKMELAGRVKVLHVFTHHVVAKSDCRVTVSIADGSVLGFHC 342



  Database: tair10
    Posted date:  Dec 8, 2010 11:30 AM
  Number of letters in database: 11,106,569
  Number of sequences in database:  27,416
  
Lambda     K      H
   0.322    0.139    0.421 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 27416
Number of Hits to DB: 3,460,734
Number of extensions: 87215
Number of successful extensions: 184
Number of sequences better than 1.0e-05: 2
Number of HSP's gapped: 182
Number of HSP's successfully gapped: 3
Length of query: 259
Length of database: 11,106,569
Length adjustment: 96
Effective length of query: 163
Effective length of database: 8,474,633
Effective search space: 1381365179
Effective search space used: 1381365179
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.9 bits)
S2: 110 (47.0 bits)