BLASTP 2.2.23 [Feb-03-2010]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Reference for compositional score matrix adjustment:
Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala,
Aleksandr Morgulis, Alejandro A. Schäffer, and Yi-Kuo Yu (2005) "Protein database
searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109.

Query= Os01g0225100 Os01g0225100|AK100398
         (309 letters)

Database: tair10 
           27,416 sequences; 11,106,569 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

AT5G58720.1  | chr5:23719976-23722974 REVERSE LENGTH=520          211   5e-55
AT3G15390.1  | chr3:5196689-5199531 REVERSE LENGTH=491             69   4e-12
AT2G26280.1  | chr2:11188143-11190579 REVERSE LENGTH=568           52   4e-07

>AT5G58720.1 | chr5:23719976-23722974 REVERSE LENGTH=520
          Length = 519

 Score =  211 bits (536), Expect = 5e-55,   Method: Compositional matrix adjust.
 Identities = 114/274 (41%), Positives = 172/274 (62%), Gaps = 15/274 (5%)

Query: 47  TDEMRNMSYNRSDNEHGFFWGE--LQRSYLK--VACEEPHXXXXXXXXXXXXXXXPQQVL 102
           +D +   S++   ++   FWG    QR Y K  ++ ++P                PQ+VL
Sbjct: 249 SDSLAESSFDTDTSDCELFWGGDYSQRDYAKALMSSQDPFATTQGIDELGL----PQKVL 304

Query: 103 DSLFKIPEQRTYEPSSMDWKKVVKRLQSFNSPITSNNQEKPK-----NGNGYQEFRTVAG 157
           +SLF I +   +E  +  W+ V K++QS     +S++ E+P        + Y E R  A 
Sbjct: 305 ESLFNIRQNPKHESKTTSWRNVAKKMQSLGIDASSSSGEEPHPNTFVKDDSYHELRKGAN 364

Query: 158 RHYDTMKTYYQKAAMAYSKGEKSYASYLAEEGKHYRELARMEDERASREIFEARNKHITN 217
             ++  K+YYQKAA AYSKG +++A+YL+++G+   + A+  DERAS++IF ARNK I N
Sbjct: 365 DQWNVTKSYYQKAAEAYSKGGRAHAAYLSDKGRVASKQAQRADERASQDIFVARNKGIEN 424

Query: 218 TVTIDLHGQHVKQAMRLLKVHMMICVCMPTT-FLRVITGCGVEGTGKGKIKRAVAELVEK 276
            VTIDLHGQHVK AM+LLK+H++    +P+   LRVITGCG  G GK K+K++V +L+E+
Sbjct: 425 VVTIDLHGQHVKPAMKLLKLHLLFGSYVPSIQTLRVITGCGASGFGKSKVKQSVVKLLER 484

Query: 277 EGVEWHEENAGTIVLRL-GGPREYRFLEHDSDSD 309
           EGV + EEN GT++++L GG RE+ FL+ +SDSD
Sbjct: 485 EGVRYCEENRGTLLIKLDGGSREFSFLDTESDSD 518



>AT3G15390.1 | chr3:5196689-5199531 REVERSE LENGTH=491
          Length = 490

 Score = 68.6 bits (166), Expect = 4e-12,   Method: Compositional matrix adjust.
 Identities = 46/163 (28%), Positives = 84/163 (51%), Gaps = 10/163 (6%)

Query: 136 TSNNQEKPKNGNGYQEFRTVAGRHYDTMKTYYQKAAMAYSKGEKSYASYLAEEGKHYREL 195
           +SN  ++  + N ++  R     H + MK YY  AA A+SKGE   A  L E+G  + + 
Sbjct: 315 SSNTSKEDDDENEFKAHRKAVREHLNQMKEYYGAAAEAFSKGETERAHRLVEKGHFFGQK 374

Query: 196 ARMEDERASREIFEARNKHITN-----TVTIDLHGQHVKQAMRLLKVHMMICVCMPT-TF 249
           AR  D+++  ++ +   +  +       VT++++    K+A+RLLK  ++    + +  +
Sbjct: 375 AREADDKSVAKMIDVNQEDDSTYEEDEVVTVNMNEHEAKEALRLLKRQLIYFSGISSFKY 434

Query: 250 LRVITGCGVEGTGKGKIKRA-VAELVEKEGVEWHEENAGTIVL 291
           LRV  G   E     K KR  + +L+E E + W EE+ G +++
Sbjct: 435 LRVQLG---EKKEDFKSKRKHIVKLLEGESIPWTEEDGGLVIM 474



>AT2G26280.1 | chr2:11188143-11190579 REVERSE LENGTH=568
          Length = 567

 Score = 52.0 bits (123), Expect = 4e-07,   Method: Compositional matrix adjust.
 Identities = 43/158 (27%), Positives = 72/158 (45%), Gaps = 14/158 (8%)

Query: 146 GNGYQEFRTVAGRHYDTMKTYYQKAAMAYSKGEKSYASYLAEEGKHYRELARMEDERASR 205
           GN Y E R  A  +      Y+++A  AY  G K+ A  L+ +G+ +    +    +A  
Sbjct: 405 GNMYSELREEARDYARLRNVYFEQARQAYLVGNKALAKELSVKGQLHNMQMKAAHGKAQE 464

Query: 206 EIFEARN-----KHITNTVTIDLHGQHVKQAMRLLKVHMMICVCMPTT-------FLRVI 253
            I+  RN         N   IDLHG HV +A+++LK  + +              ++ V 
Sbjct: 465 AIYRQRNPVGQGNSRGNERMIDLHGLHVSEALQVLKHELSVLRSTARATQERLQIYICVG 524

Query: 254 TGCGVEGT-GKGKIKRAVAE-LVEKEGVEWHEENAGTI 289
           TG    G+    ++  AV   L+E+EG+++ E  AG +
Sbjct: 525 TGHHTRGSRTPARLPVAVQRYLLEEEGLDYSEPQAGLL 562



  Database: tair10
    Posted date:  Dec 8, 2010 11:30 AM
  Number of letters in database: 11,106,569
  Number of sequences in database:  27,416
  
Lambda     K      H
   0.315    0.131    0.384 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 27416
Number of Hits to DB: 6,563,326
Number of extensions: 266741
Number of successful extensions: 788
Number of sequences better than 1.0e-05: 3
Number of HSP's gapped: 782
Number of HSP's successfully gapped: 3
Length of query: 309
Length of database: 11,106,569
Length adjustment: 98
Effective length of query: 211
Effective length of database: 8,419,801
Effective search space: 1776578011
Effective search space used: 1776578011
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.6 bits)
S2: 111 (47.4 bits)