BLASTP 2.2.23 [Feb-03-2010]
Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Reference for compositional score matrix adjustment:
Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala,
Aleksandr Morgulis, Alejandro A. Schäffer, and Yi-Kuo Yu (2005) "Protein database
searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109.
Query= Os01g0158500 Os01g0158500|J065005P06
(80 letters)
Database: tair10
27,416 sequences; 11,106,569 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
AT1G66530.1 | chr1:24819064-24822277 REVERSE LENGTH=591 130 1e-31
AT4G26300.1 | chr4:13308400-13313109 REVERSE LENGTH=643 129 4e-31
>AT1G66530.1 | chr1:24819064-24822277 REVERSE LENGTH=591
Length = 590
Score = 130 bits (327), Expect = 1e-31, Method: Composition-based stats.
Identities = 60/80 (75%), Positives = 67/80 (83%)
Query: 1 MLVCGIKTWAPILPVKRAVLDFSSPNIAKEMHVGHIRSTIIGDTLAHMFEFTNVEVLRRN 60
MLV GI TWAP L VKRAV+DFSSPNIAKEMHVGH+RSTIIGDTLA M E++ VEVLRRN
Sbjct: 114 MLVDGIDTWAPTLSVKRAVVDFSSPNIAKEMHVGHLRSTIIGDTLARMLEYSKVEVLRRN 173
Query: 61 HVGDWGTQVLNLNVLIFKQF 80
HVGDWGTQ L +F++F
Sbjct: 174 HVGDWGTQFGMLIEFLFEKF 193
>AT4G26300.1 | chr4:13308400-13313109 REVERSE LENGTH=643
Length = 642
Score = 129 bits (323), Expect = 4e-31, Method: Composition-based stats.
Identities = 58/80 (72%), Positives = 68/80 (85%)
Query: 1 MLVCGIKTWAPILPVKRAVLDFSSPNIAKEMHVGHIRSTIIGDTLAHMFEFTNVEVLRRN 60
ML+ G+ TWAP L VKRAV+DFSSPNIAKEMHVGH+RSTIIGDTLA M E+++VEVLRRN
Sbjct: 166 MLIDGVDTWAPTLSVKRAVVDFSSPNIAKEMHVGHLRSTIIGDTLARMLEYSHVEVLRRN 225
Query: 61 HVGDWGTQVLNLNVLIFKQF 80
HVGDWGTQ L +F++F
Sbjct: 226 HVGDWGTQFGMLIEYLFEKF 245
Database: tair10
Posted date: Dec 8, 2010 11:30 AM
Number of letters in database: 11,106,569
Number of sequences in database: 27,416
Lambda K H
0.328 0.141 0.444
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 27416
Number of Hits to DB: 1,652,890
Number of extensions: 52487
Number of successful extensions: 128
Number of sequences better than 1.0e-05: 2
Number of HSP's gapped: 128
Number of HSP's successfully gapped: 2
Length of query: 80
Length of database: 11,106,569
Length adjustment: 52
Effective length of query: 28
Effective length of database: 9,680,937
Effective search space: 271066236
Effective search space used: 271066236
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 15 ( 7.1 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 40 (21.7 bits)
S2: 104 (44.7 bits)